Khrisna Putera Buddhiman

11 Questions 19 Answers 0 Followers

Questions related from Khrisna Putera Buddhiman

Have you ever seen this black spot in your animal cell culture?

Dear all, My lab has just recently got a cell line. The cells grow a bit slowly and the medium color is changing quite fast from pink to orange compared to other cell lines. In addition, we...

17 October 2022 6,628 6 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

24 February 2021 2,376 2 View

Do anyone have experience in preparing Chicken Embryo Liver Cells?

Hello. Do anyone have chicken embryo liver (CEL) cell propagation in roller bottles protocol? And if anyone already have some experience in preparing CEL, do you have ever experienced "slime"...

13 March 2018 1,525 4 View

Do you have opinion of these brown spots on my PK-15 cells?

Hi all, I want to ask about my cell culture. My cell is PK-15 cell line. When they are already reach the confluency, I noticed there are several rounded cells grouped and formed brown spots. Do...

27 December 2017 9,891 5 View

Do you have suggestion about freeze drying process?

Hi, I'm trying to make freeze dried virus stock. We use lactose+pepton stabilizer. Turned out the cake formation is imperfect. Do anyone can analyze and have suggestion what can be improved from...

30 October 2017 7,003 3 View

How to titrate PCV2 Virus (TCID50)?

Dear all, I'm currently working with PCV2 virus and want to optimize the titration protocol. Currently we don't have resources to do IFA or IPMA assay, so it will be based on CPE observation...

22 May 2017 2,534 5 View

Vero cells morphological change?

Dear all, I'm currently culturing Vero cells using Eagle MEM+FBS media. A few days ago, I noticed some morphological change in my culture. The cells are getting bigger and they formed circular...

06 February 2017 977 9 View

What happened to my Vero cells?

Hi I'm just culturing Vero cells from liquid nitrogen stock. The growth is slow. We use EMEM with 10% FBS. By looking at the micrograph (especially cell shape and artifacts), is there anything can...

20 January 2017 1,947 6 View

Is hyperthermia possible using clinical MRI?

Hello, I'm currently working in magnetic hyperthermia related topic. Is it possible to achieve temperature rise if I put iron oxide magnetic particles in a RF coil of 1.5 T MRI (64 MHz) for 5...

08 August 2015 4,186 2 View

What's the reason behind SKOV3 cells shape change?

I'm trying to culture SKOV3 cells with RPMI 1640 medium+10% FBS+insulin+PenStrep. Something unusual happened after my first splitting with 1:2 ratio using Trypsin & EDTA. The shape of the...

30 January 2015 3,637 4 View

Could you share your experience on increasing bacterial cfu and biomass yield on fermenter?

Hello all, I'm quite new to this topic of research. I'm seeking advice on how to increase bacterial CFU and biomass yield on a fermenter level. The bacteria is one of the Bacillus species. The...

01 January 1970 4,677 7 View