I am currently working on a germination assay to compare three genetically distinct genotypes, including native and positive control lines. I am scoring seed germination every day for seven days under several stresses.
I would appreciate it if anyone could share their experience and recommendations regarding:
- The best practices for scoring germination( what expected to see or count)
- What criteria should be used for counting a seed as "germinated"
Any insights, tips, or examples from your work would be really helpful. Thank you in advance!
Yes, germination assays in Arabidopsis thaliana under stress conditions (such as salinity, drought, temperature extremes, or oxidative stress) are widely used to study plant stress tolerance. Researchers monitor germination rate, seedling vigor, and survival to evaluate genetic or chemical treatments. These assays help identify stress-responsive genes, signaling pathways, and potential targets for crop improvement.
Germination is the emergence of any plant part from the seed :)
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