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Questions related from Maryam Mz
Can I sow seeds after keeping them in the dark for only one day instead of the usual 2–4 days following surface sterilization?"
08 May 2025 7,376 2 View
I am currently working on a germination assay to compare three genetically distinct genotypes, including native and positive control lines. I am scoring seed germination every day for seven days...
19 April 2025 4,776 3 View
I need help with a process and protocol to measure chlorophyll content in Arabidopsis seedlings grown in (-N) media. I also need guidance on how to perform the calculation.
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Could you share your experiences with eluting plasmid DNA? According to the protocol, the binding column is transferred to a clean 50 mL collection tube, and 3 mL of Elution Solution (or molecular...
23 February 2025 8,721 4 View
I was wondering if anyone has experience working on ER stress in Arabidopsis plants? I would really appreciate it if you could share the concentrations and volumes you used to measure ER stress or...
19 January 2025 2,277 2 View
Dear all, I am trying to get a homozygous from Sail line stocks. Plants are the third generation from a homozygous line, and I am still unable to detect any clear or correct bands. It seems that...
11 January 2025 8,206 5 View
I have a question regarding the phenotypic stress assay. If I am conducting salt stress testing on three genotypes (including WT) under different salt concentrations ( e.g: 50mM, 100mM and 150mM)...
01 January 1970 2,845 5 View
