Currently we are using the petioles for transformation In-Vitro. I am interested if somehow we can manage to shift to In Planta transformation as it is an easy and cost effective process.
https://www.researchgate.net/publication/315774847_Agrobacterium_Mediated_Gene_Transfer_make_Disease_free_plants
https://www.researchgate.net/publication/6557580
Article Agrobacterium-Mediated Gene Transfer Results Mainly in Trans...
Dear sir i asked for in Planta Transformation but the material shared does not have the In Planta related information. Thank you
Dear friend
I'm not clear about 'planta transformation but I'm interested in ATMT
See this it may helpful to you
https://protocols.scienceexchange.com/protocols/in-planta-transformation-of-brassica-rapa-and-b-napus-via-vernalization-infiltration-methods
I have seen only one publication using in planta transformation in Brassica. To my knowledge this method has not been confirmed by other labs.
I think it is this one:
Plant Cell Rep. 2003 Nov;22(4):274-81. Epub 2003 Aug 29.
Development of a novel Agrobacterium-mediated transformation method to recover transgenic Brassica napus plants. Wang WC1, Menon G, Hansen G.
Dear Rahil,
I would recommend you below mentioned link. Though its citrus but try to improvise it for Sunflower. I am sure, it will work
http://www.sciencedirect.com/science/article/pii/S0304423816306021
Good luck,
Allahbakhsh