Hat happiness when change ratios salts of iron to produce iron oxides nanoparticles?

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How to choose the stimulus for T-cell activation assays?

When doing T-cells activation assay, for example looking for CD25 ,CD69 expression or ELISA for cytokine release, is there a difference between the coated antibody or coated protein stimulation...

08 November 2019 836 3 View

What is the function of OM-1 in gene expression?

Hello I have a plasmid to transduce on T-cells. The sequence of this plasmid contains OM-1, which I know only it helps for protein expression, but how does it work I couldn't find an explanation...

02 September 2018 4,617 1 View

What are possible reasons for tumour cell line not killed by T-cells in vitro?

Hello, I work in cancer immunotherapy particularly in CAR T-cells. I have leukaemia cell lines such as KO-52, ksaumi and THP-1. They express my target antigen at different levels. My CARs are also...

19 June 2018 1,729 2 View

Why are the results variable between wells of same samples in protein assay?

Hello there, I'm doing protein concentration for WB assay using BioRad DC™ Protein Assay similar principle to Lowry assay. I have 8 samples and standard.I do them in triplicates. However, I've...

10 May 2018 6,529 8 View

Should I do CD34 isolation of primary AML cells before flow staining?

Hello I have AML cells that have been cultured on stromal cells for weeks and would like to do flow staining looking for a specific antigen in the AML cells. I am not sure where these cells came...

28 January 2018 4,918 2 View

What are the cell lines that have intermediate and low levels of CD19 Expression?

Hello I am working on CD19-directed CAR T-cells and using CD19 expressing cell lines for cytokine and killing assays. I want to include different cell lines that have different levels of antigen...

15 June 2017 721 2 View

What points should I consider to design a primer for DNA sequencing ?

HelloI did restriction digestion and ligation of a huamn gDNA construct and I have now the pure plasmid DNA and want to check the sequence alignment.I've read the things that should be considered...

11 September 2016 1,093 3 View

How can I choose analytical function or model profile fitting in TOPAS?

There are many types of analytical functions and model profile fitting that we must choose during refinement using TOPAS programme. The model profile fitting include ; 1) FP - Fundamental...

11 August 2015 9,440 4 View

Was the size play a main role for the poling process in piezoelectric material?

I am now working on lead free piezoelectric material with barium titanate (BaTiO3) based material. However, I faced problems while doing poling process. I used to pole my sample in pallet form...

25 September 2014 7,484 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

Why chloride turns into chlorine gas in a Downs cell?

I imagine the battery drives the graphite into a carbocation, then chloride is oxidized to donate the electron to the carbocation, but what pushes the lost electrons of chloride to be shared...

03 August 2024 5,384 0 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

How to select target material for X-Ray generation?

Generally, the target material is Mo, Co, Cu, Fe, Cr, etc, so on what basis should we choose this material?

31 July 2024 5,824 0 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Thomas Walther

sorry, your question is not intelligible - please rephrase

Yuri Mirgorod

Huwaida Bahashwan

If you take your desired ratio, you will get magnetite FeO•Fe2O3. Magnetite has a favorable crystal lattice with a minimum of Gibbs energy, with certain stable magnetic properties. If you change the ratio, the formation of a favorable crystal lattice will not be. A mixture of oxides FeO and Fe2O3 is formed.

Run Xu

i don't know