We bought OxPAPC, but we don't have nitrogen or argon for the chloroform resuspension. Has anyone tried resuspending it in medium? How much time can it be stored?
Thanks! Finally I resuspended half of it in DMEM, as indicated and stored it in the freezer. I hope it remains stable...did you check the stability?
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
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Hi! I ran different models using the glmer function in the lme4 package and compared the performance of these models using the compare_performance function in the performance package. The model...
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We bought a bottle of Penicillin G potassium salt 10 MU and we do not know what kind of water we need and how much of it to dissolve the antibiotic. Thanks so much
06 August 2023 7,446 2 View
I've been battling with codes and it's no use. There must be a way to select a group of taxa as the outgroup, but I couldn't find how. I suppose that the alternative path is to make the whole...
22 June 2023 9,302 2 View
Hello, I prepared 4 mg/mL corticosterone in sesame oil for subcutaneous injection in mice. To do so, I had to sonicate and warm up the solution to facilitate corticosterone solubility. I was...
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when men are forced to be fathers when they were tricked by women to get pregnant. and studies for men's rights
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Guillermo Zalba is not part of the team of the article, plese delete his name Best regards Alejandra Guillermina Miranda-Díaz, MD, PhD
01 March 2023 7,262 2 View
I am trying to obtain chitin by a chemical method. After deproteinization and demineralization processes, the powder obtained after each step is too alkaline or acidic respectively. Then, I spend...
10 July 2022 1,686 3 View
Good morning, I wrote if someone can give me a solution to a problem that afflicts my culture of chlorella vulgaris. Lately I have noticed an excessive formation of foam at the top, and this foam...
06 April 2022 972 0 View
Good morning, I have a question and I hope someone can help me. I have as inoculum chlorella vulgaris, with photoperiod of 12/12 hours, shaker agitation up to a volume of 500 mL and agitation...
21 February 2022 970 0 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
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How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
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Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
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Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
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kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View