How to check normal distribution in a cell viability test?

To choose whether a parametric or a non-parametric test should be used to compare data on viability that emerge from an assay such as MTT, one should establish if the data are normally...

02 February 2021 1,733 4 View

Why I observe different growth curves in Flp-In T-REx system?

Hi, I'm currently working with the Flp-In T-REx system to get isogenic stably transfected cells expressing my GOI (gene of interest, of about 1.4 Kb). I have generated a Flp-In Cell Line and since...

17 February 2020 5,608 2 View

Why does my MS show me only base peaks?

After changing the septum of my Focus GC connected to a DSQ II MS and after a complete cleaning operation on it, my mass spectrum became completely random. It shows me only base peaks in a...

10 June 2019 481 4 View

Can anyone suggest key literature and leading institutions in the field of Energy Transition?

Dear all, My background is in Energy Economics and Political Science and I would like to improve my knowledge on the Energy Transition issues. In your opinion, what are the most relevant and...

25 October 2018 8,507 7 View

How to simulate 3D beam to 3D beam contact in ansys APDL?

Hi, I'm modeling a lattice structure and I would like to insert the contact elements between 3D beams to get the whole structure non linear deformation. Since I never used contact elements before...

22 July 2018 1,551 2 View

GNSS Carrier phase Single difference residuals?

Dear all if I have the SD carrier-phase measurements how can I compute the SD residuals? Do i need to calculate the SD residuals before fixing the integer ambiguity?

10 April 2018 7,001 2 View

Interpolation of product formation in biocatalysis using integrated Michaelis-Menten equation?

I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...

15 January 2018 6,790 3 View

What is the main goal of modeling, and maybe of scientific knowledge tout court?

While ideally aiming at understanding an empirical phenomenon, and at the same time predicting its behaviour, scientists often focus only on one of these two aspects. Is this the dilemma of a...

13 November 2017 6,036 5 View

Are there physical applications of Banach-Tarski Paradox?

Banach-Tarski Theorem (also known as Paradox) is a mathematical statement which says that a sphere can be splitted into two (or an integer N...) spheres each equal to the original one. Do you know...

30 October 2017 9,335 2 View

What is the state of art regarding Navier-Stokes?

Which is the status of mathematical research about existence and unicity for Navier-Stokes equations (and boundary problems) for finite or infinite domains? Gianluca

17 October 2017 1,618 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View