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Questions related from Gianluca Occhi
Hi, I have established the best transfection condition that maximizes the silencing effect of Dharmacon siRNA (with Dharmafect-1) on my gene of interest (GOI). My study aims to evaluate the effect...
13 September 2021 2,202 4 View
To choose whether a parametric or a non-parametric test should be used to compare data on viability that emerge from an assay such as MTT, one should establish if the data are normally...
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Hi, I'm currently working with the Flp-In T-REx system to get isogenic stably transfected cells expressing my GOI (gene of interest, of about 1.4 Kb). I have generated a Flp-In Cell Line and since...
18 February 2020 5,849 2 View
I hope somebody is able to give me suggestions. After treating a 50:50 mix of fully methylated (FM) and non-methylated (NM) commercial DNA (zymosearch) I amplified the locus I want to study. Once...
09 May 2017 4,863 2 View
There are published evidences of differential methylation pattern in a specific gene hosting locus I'm interested in, between different grade tumors. These data were obtained using the HumMeth450...
09 November 2016 5,363 4 View
Has somebody experience in blunting plasmids' sticky ends using a Phusion High Fidelity (NEB)? Suggested protocols?
12 October 2015 8,509 2 View
Can somebody help me to understand how can I translate a drug dosage used in cultured cells to that that should be used in humans. Does it depend in some way on the molecules I'm working with and...
22 November 2013 2,383 4 View
I plan to silence a hypothetical tumor suppressor gene in two cell lines. I was first convinced that a stable transfection with shRNA was best, although at the beginning it requires more work....
30 August 2013 8,680 23 View
Dear all, I am studying a genomic locus in some tumoral specimens with the aim of clarifying if there are differences in the methylation status of about 30 CpGs among different samples (two groups...
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