Hi all,
I'm trying to investigate whether cell-free fetal DNA has been detected in peripheral maternal tissues other than plasma/serum. Does anyone know of research considering saliva?
We are trying to use flow cytometry to detect cell viability / apoptosis to determine IC50 values after exposing adherent cells to drugs. We are concerned that during the washing / processing...
23 June 2024 4,279 0 View
Mother's mental health condition was included in cut and paste over to baby's chart and released without her authorization.
13 March 2024 3,362 0 View
I have an essay to write on the comparative method in linguistics I have to discuss the purpose and application of the comparative method in linguistics, emphaising its role in protoforms, state...
27 February 2024 7,681 0 View
Dear colleagues, Has anyone of you ever experienced difficulties with the high background of the solvent-PBS (used to suspend the exosomes after isolation by ultracentrifugation) during NTA...
09 January 2024 7,735 3 View
All approved medical treatments for osteoporosis have risks associated with them. Has anyone actually researched the idea of restarting HRT after patients have tried other therapies?
23 November 2023 4,719 0 View
Cytiva sells a GST antibody as part of a rather expensive kit for coupling tagged proteins to their sensor chips. The antibody can not be purchased separately, and we do not need all the other...
26 October 2023 3,800 1 View
I have been performing thermal melt analysis of a protein that can form dimers or other higher order structures. Thus, we were not too surprised to see a melting profile that seems to have two...
22 October 2023 9,445 0 View
I have two IVs with 4 groups each and two DVs that are continuous. What statistical test do I use? Thank you.
20 July 2023 157 4 View
I've been trying to activate and expand naive T cells that have previously been isolated from PBMCs with a pan-T cell isolation kit from Miltenyi and cryopreserved in 90% FBS 10% DMSO. I have...
10 July 2023 9,088 2 View
I have recently managed to have Devine's Master's thesis on Curtius posted on the UQ espace, but access is limited to current students and staff. I'd like to see it made more widely available,...
16 November 2022 5,875 7 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View
Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...
11 July 2024 8,115 2 View