How do i search for a dissertation?

I was referred her because i wanted to ask the author to view the dissertation The Housing that Model Cities Built: Context, Community and Capital in New York City, 1966-76, Schindler Killian...

16 December 2020 5,849 6 View

How to prepare a 0.2 M phosphate buffer?

I need to prepare a 0.2 M phosphate buffer. In my research, I've seen people prepare it with both monobasic and dibasic sodium phosphate... is this necessary? Can I just use one or the other?

13 September 2020 4,656 2 View

Non-parametric equivalent of three-way ANOVA for small sample size?

I inherited a dataset with a very small sample size (n=3 per group) and three factors (primary insult x secondary insult x treatment). I intended to use a three-way ANOVA, but I was warned that...

31 March 2020 5,913 3 View

How do I obtain a doi for my publication?

A researcher here at my institution is asking about getting a doi for his publication to increase its findability and exposure. Does one have to go through the avenue of a Registration Agency? If...

04 February 2020 8,760 6 View

Does genomic DNA bands smear on gel are degraded DNA?

I have to send my sample for metagenomics sequencing but my genomic DNA shows up a clear band with thin and long smear. It looks like my sample has degraded but I'm not sure. I read somewhere that...

15 December 2019 206 3 View

How to summarize reliability values ?

Hello, I am coding some metrics from different articles to run a meta-analysis and I had a simple question. Let's say one of my variable of interest is Brand loyalty. In some articles, brand...

19 August 2019 171 2 View

Ways to dry phenolic extracts in 70% acetone (2mL samples in 96 deep well plates)?

We are extracting phenolics using 70% acetone. The samples are set up in 96 deep well plates. The CentriVap (rotary vacuum) and lyophilizer that we have are made with materials that could be...

02 September 2018 3,880 3 View

Was the heavy cavalry of Alexander the Great the first cavalry to charge home in combat against unbroken enemy cavalry?

18 August 2018 7,200 6 View

Methods for sterilizing (killing biota on) granular activated carbon (GAC) ?

I am only interested in methods that don't change the GAC's surface chemistry or absorption capacity. The ionising and non-ionising irradiation methods we have looked into seem likely to affect...

03 April 2018 5,313 3 View

Probability of viral plaques to skip a dilution at low levels?

Hello all, I'm looking to calculate the probability to have viral plaques appear in lower dilutions but not in the previous dilution. We perform serial 10 fold dilutions (0.2 ml into 1.8ml...

20 February 2018 2,350 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View