I bought the ZF4 cell line from ATCC. The cells were frozen in liquid nitrogen before any other steps. I am cultivating them for two weeks now and the cells didn't have any improvement. I'm using DMEM: F12 plus FBS (10%), and trypsin without EDTA, the cells are incubated at 28 °C in an atmosphere with 95% O2 and 5% CO2, as suggested by the ATCC. Also, there's so much debris in the medium all the time. Can anyone help me?
Hi Prisicila, I have just read your comment from 2023. I bought the same cells and used the same conditions. I observed low growth during the first 3 weeks more or less. It is important to cultivate them in small flasks at the beginning, be patient and centrifuge them at least at 1000 rpm to obtain a precipitate before changing the flask
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