I am wondering if anyone has used and characterized the expression of Cre in mature astrocytes vs neuronal cells using the B6.Cg-Tg(Gfap-cre)77.6Mvs/2J line of GFAP-Cre driver? http://jaxmice.jax.org/strain/024098.html
It seems that inducible lines have issues getting complete astrocyte coverage (and determining the right timing and dosage of tamoxifen) and non-inducible lines hit progenitors and neuroblasts early in development resulting in too much expression in neurons. The aforementioned line is relatively new, so I was wondering if anyone had characterized it as yet?
Hi Natalie
B6.Cg-Tg(Gfap-cre)77.6Mvs/2J seems to be the best so far. 73.12Mvs/J is not good, as it expresses Cre in adult neural stem cells.
In B6.Cg-Tg(Gfap-cre)77.6Mvs/2J, 80-90% of the cortical and hippocampal astrocytes express Cre. Cre expression in astrocytes in other brain regions is relatively sparse. Important to note, there is also very sparse neuronal expression of Cre in the cortex of P21 mice.
Good luck
Boaz
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