Natalie Prowse

5 Questions 4 Answers 0 Followers

Questions related from Natalie Prowse

Recommendations for decent antibodies for CREB/pCREB in Western Blots?

Hi, I'm having problems finding CREB and pCREB antibodies that tag the same proteins in my blots. (see attached) We are testing between 5 and 15ug protein loads from mouse hippocampal cell...

23 January 2022 200 1 View

Elisa kit recommendations for CD3 detection from frozen spleen samples?

Hi, I have some flash-frozen mouse spleens (in the -80C) that I'd like to analyze for CD3, preferably using an Elisa kit to reduce time. I've been searching various sites, but not a lot of options...

23 October 2020 8,792 2 View

Is this yeast contamination in primary glial cell culture?

This is a primary glial culture (taken from pups @PD2). Picture taken at 10DIV. Astrocyte layer is completely confluent. We are actually after the microglial layer underneath the astrocytes...

04 April 2020 9,036 1 View

Has anyone used the Miltenyi myelin removal beads with the EasyStep magnet/system?

We already have the EasyStep magnet system from StemCell Technologies. We have used it for IP of specific receptor proteins using Thermofisher/Invitrogen Dynabeads and commercially available...

01 October 2019 8,861 1 View

How to normalize western blots between membranes?

On studies with a high number of treatment groups and/or animals, it takes several gels to run all our samples. We always run duplicate gels, and we have noted that even between gels with the...

04 November 2016 9,925 8 View