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Questions related from Natalie Prowse
Hi, I'm having problems finding CREB and pCREB antibodies that tag the same proteins in my blots. (see attached) We are testing between 5 and 15ug protein loads from mouse hippocampal cell...
23 January 2022 211 1 View
Hi, I have some flash-frozen mouse spleens (in the -80C) that I'd like to analyze for CD3, preferably using an Elisa kit to reduce time. I've been searching various sites, but not a lot of options...
23 October 2020 8,826 2 View
This is a primary glial culture (taken from pups @PD2). Picture taken at 10DIV. Astrocyte layer is completely confluent. We are actually after the microglial layer underneath the astrocytes...
04 April 2020 9,073 1 View
We already have the EasyStep magnet system from StemCell Technologies. We have used it for IP of specific receptor proteins using Thermofisher/Invitrogen Dynabeads and commercially available...
01 October 2019 8,871 1 View
On studies with a high number of treatment groups and/or animals, it takes several gels to run all our samples. We always run duplicate gels, and we have noted that even between gels with the...
04 November 2016 9,936 8 View
I am wondering if anyone has used and characterized the expression of Cre in mature astrocytes vs neuronal cells using the B6.Cg-Tg(Gfap-cre)77.6Mvs/2J line of GFAP-Cre...
21 April 2015 9,874 2 View
I'm looking for a as simple as possible protocol for the quantification of polyamines such as spermine, spermidine, putrescine and agmatine, and their metabolites from blood plasma and brain...
07 February 2015 8,050 7 View
