Has anyone used stationary 3D cameras to reconstruct mesh images of moving objects, and derived morphometrics from them?

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What to do about some data sets being normally distributed, some being normally distributed after log transformation and some not norm. dist. at all?

Hi all, I have collected some data from 3 treatment groups (n=5) with relation to 6 markers of fibrosis. When I was looking to analyse (with GraphPad Prism) these 6 data sets, I noticed that some...

11 April 2024 7,526 3 View

Medical lab quality/risk professionals - do you use medical goals? Will you share?

Since 1999, the recommended source of allowable error limits or Analytical Performance Standards has been to set limits based on medical need in specific clinical settings. I have been doing this...

09 April 2024 7,424 2 View

What is wrong with my logistic regression analysis?

I have a problem with running my logistic regression. When I run my analysis, I get really strange values and I cannot find anywhere how I can fix it. I already changed my reference category and...

09 March 2024 5,668 3 View

Issues with DNA extraction A260/A230 in plants?

I'm doing DNA extractions on johnsongrass and the 260/230 ratios are extremely low (highest I got was 0.5). I'm using the Qiagen DNeasy Plant Pro Kit and I already tried leaving the AW2 buffer for...

28 February 2024 2,009 0 View

Substituting RT in Illumina mRNA Library Prep Kit with Superscript III ?

Ran out of RT in Illumina mRNA prep Kit, unfortunately the company doesn't sell it separately; plan to substitute it with Superscript III. Does anyone done that before? Will it work?

20 February 2024 1,284 0 View

Why did the silver industry in Connecticut attract Puerto Rican labor?

Looking for research on 20th century immigration to CT for the purpose of working at International Silver in Meriden CT

19 February 2024 9,028 0 View

How will using ammonium acetate instead of formic acid in water mobile phase on HPLC affect LCMS results?

Hello, inexperienced grad student here. I've been processing samples via HPLC/LCMS (tissue extractions from plants) over the past year or so. We have two mobile phases that we use in a shared HPLC...

26 June 2023 6,046 3 View

Calculate battery energy density only with the specific capacity of anode and cathode?

Oftentimes research papers on batteries will mention the capacity and the resulting energy density of a battery system in the background, but I'm confused how the theoretical energy density is...

05 April 2023 1,945 2 View

What would the complications be for a person with a rare blood type in a medical emergency?

Compared to those with common blood groups, what struggles does a person with a rare blood group have to go through during medical emergencies such as lack of universal blood type? Although...

22 February 2023 1,143 2 View

How to modify the surface of AuNPs with HS-PEG-NHS without NHS hydrolyzing?

Trying to PEGylate gold nanoparticles with HS-PEG5000-NHS

01 September 2022 5,939 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View