Has anyone used standard Polymerase in a long-RT-PCR?

More Edgar Rogelio de la Rosa-Marban's questions See All

Which bacterial strains should I use for phiX174 production?

Hi again, I'm looking for help finding the protocols and details involving bacterial strains for bacteriophage phiX174 production. Any knowledge, details or experience is appreciated. Thank you...

23 February 2015 9,095 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

Taryn B Hogan

How much enzyme are you using? You could try increasing the volume.

E. Rasp

Have you tested your cDNA (like with referencegene)? Quality of your RNA?

Up to 1Kb is possible. Maybe increase input Taq a little, but don't overdo. Try to increase the extensiontime. Also important is MgCl2-concentration. And sometimes standard Taq just won't give the results you want. Taq can be expensive, but when trying over and over again with no results is also expensive. In a normal DNA-PCR we got very good results (1700 bp) with QiaGen Hot Start Taq DNA polymerase.

Hung King Tiong

Are these primers binding to the exon regions of your gene mRNA cDNA of study?

If the primers are binding to the exon regions, then use 1 min extension time to produce 1kb amplicon.