Yes I have used the cryosections (30-20 micron) of rat brain for fluorojade stain. Just follow this protocol.

The sections were washed with water to remove PBS salts then  typically mounted on 1% gelatin coated slides and then air dried. The slides were first immersed in a 80% alcohol for 3 min. This was followed by 2 min in 70% alcohol and 2 min in distilled water. The slides were then transferred to a solution of 0.06% potassium permanganate for 10 min, preferably on a shaker table to insure consistent background suppression between sections. The slides were then rinsed in distilled water for 2 min. The staining solution was prepared from a 0.01% stock solution for Fluoro-Jade B that was made by adding 10mg of the dye powder to 100ml of distilled water. To make up 100ml of staining solution, 4ml of the stock solution was added to 96ml of 0.1% acetic acid vehicle. This results in a final dye concentration of 0.0004%. The stock solution, when stored in the refrigerator was stable for months, whereas the staining solution was typically prepared within 10 min of use and was not reused. After 15 min in the staining solution, the slides were rinsed for 1 min in each of three distilled water washes. Excess water was removed by briefly (about 15 s) draining the slides vertically on a paper towel. Then dry the slides in dark for overnight at RT.

Then because it is the fluorescence stain, don’t mount it with DPX after xylene.

But after drying mount it with antifade and fluorescence mounting media prepare from glycerol.

Unfortunately, this is a problem with doing this stain on tissue that wasn't thaw-mounted onto slides right from the cryostat.  One thing that has helped me (slightly) is 1) making sure that there is as little water trapped between the tissue and slide as possible (i.e. when you mount, go back to each slice with the edge of a kimwipe and try to remove as much water from the slice as possible by just touching the kimwipe edge to the edge of the tissue), and 2) let the tissue dry onto the slide overnight at room temperature.   It might be worth trying plus slides instead of gel coated slides, the tissue might stick better.

Sergio, 

I agree with Kate's suggestion.  I have used FluoroJade on similarly prepared and sectioned tissue (though mine is slightly thicker at 30 or 40 microns) which I kept in cryoprotectant for long term storage and and then rinsed in TBS and mounted to SuperFrost Plus slides.  I have not had any issues with wrinkling or detaching from the slides when I ensure that they make good contact with the slides during mounting and let the sections dry completely.  

Thank you all for helping me.

I have left the slides drying overnight and then I have applied the PFA vapor before doing the protocol.

The result is perfect.

Sergio, 

That's great!  Can you tell me the protocol for fixation with PFA vapor?  I'd like to try that!

To be honest, I improvised a little, Kate.

I put the slides (face up) on a grid in a sealed plastic box with some PFA at bottom. Then, I left it for 30 min inside the stove at 37ºC.

Finally, I took the slides and sarted the staining protocol.

I made the staining with some preparations with and without PFA, and the PFA slides gave better results.

You can email me for future questions as our lab has developed Fluoro Jade B and C.

Hello everybody! I guess I'm late with this answer... Anyway, I faced with the same problem and I solved it by adding several drops of acetic acid buffer to the all ddH2O steps. And it gave perfect results. dH2O alone may make sections come off slides easy. Probably, the problem is that water has high surface tension, which can cause folds, "bubbles" or minor wrinkles. Acetic acid buffer helps to reduce the surface tension in water.

One other tricky way to escape folding and floating is that you could do the staining procedure using pasteur pippette( dropping method). There by you add jus the right amount and control swelling and folding.