Can anyone provide the complete guidelines for drug development for topical formulations for wound healing?

We have evaluated the wound healing potentials of different compounds at particular concentrations in laboratory animals. Now, we want to move in the direction of drug development for some topical...

31 December 2019 7,095 1 View

How to determine the antioxidant effect of our nanoparticles by DPPH method?

We want to determine the antioxidant effects of our prepared formulation of nanoparticles by DPPH method. Our formulation is having some col and different excipients/vehicles. Please also tell how...

09 October 2019 9,228 2 View

What is the difference / significance between western blot and IHC for assessing same growth factor/other marker (e.g. TGF-beta1, alpha-SMA, VEGF)?

I am interested in assessing the expression of some growth factor (e.g. TGF-beta 1) or other marker (e.g. PCNA, CD31). I want to know the difference in term of significance for assessing the same...

02 March 2018 6,697 3 View

Can anyone provide me the reference of Wilson's formula for % wound contraction?

I want to measure the % wound contraction from the data of wound area by using Wilson's formula. Can anyone provide me the formula and reference for this formula?

05 June 2016 6,270 0 View

Can anyone suggest or provide PDF file of books on dose selection of herbal drugs and their formulation development?

I want to mix two and more than 2 herbal drugs for the preparation of herbal formulation. Can anyone suggest or provide me the PDF file of books on herbal dose selection, their standardization in...

04 May 2016 4,262 0 View

Can anyone suggest or provide PDF file of books on wound healing?

04 May 2016 6,808 1 View

Is DMSO safer for wounds?

I want to disolve drugs in DMSO and want to apply them topically on wounds, but some researchers have pointed me that it is cytotoxic even at lower concentration i.e. 10% or more. Can anyone...

03 April 2016 1,196 8 View

Does DMSO has adverse effect on topical application on wound or healing tissue?

I am using DMSO as a vehicle for dissolving the drug for the the topical application on wounds. Dose anyone have observed the adverse effect of DMSO on wound healing or other side effects?. What...

03 April 2016 4,096 5 View

Is tri-sodium citrate used for prepration of citrate buffer dissolving streptozotocin?

Citrate buffer is used for dissolving the streptozotocin for diabetes induction. Is the tri-sodium citrate used for tie preparation of citrate buffer?

03 April 2016 8,399 2 View

Can we use dried/lypholozed methanolic extracts of plants for feeding to animals?

I have prepared some plant extracts in different solvents and among them methanolic extracts are showing best in-vitro results for antioxidant potentials. Now, I want to feed or administer these...

01 February 2016 9,794 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View