I would like to extract total nucleic acid from whole blood and bone marrow tissue using QIAsymphony DSP kit for RT-PCR .As per QIAsymphony DNA Blood handbook (Page 16), "QIAsymphony DNA Kits copurify RNA and DNA if both are present in the sample. If RNA-free DNA treatment is required, add RNase A to the sample before starting the procedure". So theoretically it should work, but am wondering if the RNA quality would be affected which will affect the downstream applications.
Hi i have only tested the traditional kits from quiagen but searching the net came this up. Take a look on it
https://www.sciencedirect.com/science/article/abs/pii/S1355030615000064
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3194047/
https://journals.sagepub.com/doi/full/10.1016/j.jala.2009.07.006
I ve read the manual seems promising versus the manual spin which i ve tried before. The main reason they came up with this idea was for eliminating the many steps which introduced errors upon the results. In the manual silica column there were errors in the binding . I see why you are asking. I hope testinomials of the kit will provide some more guidance through their applied results and not theoretical background.
Best of Luck !
Kat.
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