Has anyone tried to study cholesterol trafficking at the lysosome? Either via microscopy or any biochemistry experiments?

24 February 2023 1 9K Report

I am currently looking for experiments that I could use to track cholesterol accumulation or trafficking in and out of the lysosomes? I tried using BODIPY-cholesterol as a probe but it can be fickle and did not help us much... Any help or references would be appreciated!! We don't have access to equipment that would allow us to measure this using radioactivity

Nicolas Barois

Hello,

Filipin is my first thought but You may have a look to this article for more probes:

Article Analysis of Cholesterol Trafficking with Fluorescent Probes

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