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I am currently looking for experiments that I could use to track cholesterol accumulation or trafficking in and out of the lysosomes? I tried using BODIPY-cholesterol as a probe but it can be...
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I am currently working with COS-7 cells. I have noticed recently that every time I passage them, I find a lot of dead cells 2 days later. I use DMEM with 10% FBS and trypsinize them for 5 minutes...
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