During my PhD internship I induced an acrosome reaction in bull sperm using Calcium Ionophore and assessed it with both flow cytometry and Spermac. I read some articles about induction of acrosome reaction using progesterone in other species and I would like to try something similar in bulls and assess it with Spermac Stain.
Kind regards
Dear Andra,
We use a combined viability + acrosome staining as e.g. Ca-ionophore is very toxic causing false acrosome reaction.
With best regards: Andras
Hello,
I have used this technique during my internship. In Sweden I had access to a very performant flow-cytometer. We also did a dose-response curve for the ionophore and observed some effects related to the false acrosome reaction. I am planning to use ionophore for induction and Spermac for assessment. I also read something about using progesteron for acrosome reaction induction. Have you used it in your experiments?
Hello Andra,
We used phosphatidylcholine liposomes 25 years ago at the Cornell University. Later in Hungary we observed the spontaneous real and false acrosome reaction in liquid stored ram, and cryopreserved-thawed dog samples. My Ph.D. student, Csilla Budai is working with ram semen using different techniques - flow, fluorescent and light microscopy. Maybe you could contact her: [email protected] She has a cooperation with The Banat's University of Agricultural Sciences and Veterinary Medicine in Timisoara and her Romanian is improving.
With best regards: Andras
I am interested in knowing more about using progesterone to induce acrosome reaction. Has anybody tried that with rats?
We are a Nanotechnology company that has developed fertility treatments for sperm. We collect and deplete moribund sperm and enrich for viable sperm and or Gender. We have worked with a variety of species; bovine,Equine,porcine,human, canine dolphins, etc. . Let me know your interest in working with us.We are looking for partners to help optimize this technology as well as other aspects of fertility. [Fetal cell isolation and enrichment]
Henry
F.O. Awobajo, we are currently trying to assess the induced acrosome reaction using progesterone in bulls and boars. If you already found information needed but you still some additional things, let me know!
Hello Andra, During my PhD (2000, in Bristol-UK), we used *Ca-A23187* for the induction of AR dogs (other than bulls and rams). In the literature, A23187 have also been used in horses an rabbits. Further, chondroitin sulphate and heparin have been used in bovine with success too. However, to my knowledge, P4 has mostly been used in human. But, no trial I've seen in bulls with p4-triggered AR. Hence, it would be very interesting to see the response of bovine sperm against the P4 stimulation for undertaking AR. (For further details, you may further check my pub-list on the RG with full-text provided, as you wish). Good luck with your endeavours. Regards. Omer
Just to add, during our trials, A23187-induced AR's could have been well monitored by different methodologies; 1) PC-Phase contrast microscopy, 2) DIC-Differential Interference Contrast microscopy, 3) NA-Naphtol Yellow S plus Aniline Blue staining, 4) SEM- Scanning EM and 5) TEM-Tranmission EM. Good luck.
Hello,
I used A23187 for AR of bull sperm and assessed the reaction using flow-cytometry but I read many articles regarding P4 thus I was curious if someone obtained results this way. I am planning to make a trial next month and see how it goes. Thank you for the info and have a wonderful scientific year!
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