We recently bought this membrane for Western blot analysis and it seems that it is two-sided (one smooth and one rough). There are no details on how to use it. Is there a preferential side that should be in contact with the gel? The lab is also experiencing some problems in transfer, so I was hoping someone could explain the issue. Any suggestions?
the smooth side is the surface with PVDF. for future purchases, in my opinion, millipore PVDF works much better!
I was using Millipore before but I tried this one as it promised good results and it was less expensive.
Thanks to all for your imput. We are actually now getting good results with this membrane, although we are not able to reprobe it with other antibodies (it just doesn't work) in contrast to several other brands
be carefull, if you are using secondray ab coupled with IR dye (680-800), there is a special pvdf mb you should use, see odyssey manual. it is
Millipore Immobilon® FL PVDF Membrane very important
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