The work was just been published in: http://www.nature.com/nature/journal/v505/n7485/full/nature12968.html , and it is a very exciting piece of work. Looks like the small molecule is the way ahead for making iPSC-like cells.
This has the potential to be a huge breakthrough. In 2012, Gurdon and Yamanaka won the Nobel Prize in Med/Phys for a similar discovery in iPSCs. Except these STAP cells can form placental cells, which is where the ES/iPS were falling short.
Protocol wise, I would imagine that you could induce these cells in more than one way. pH-induced might be the easiest but it would be interesting to experiment with plasma membrane disruption, decreased O2, growth factor deprivation, increased Ca++ exposure. Identifying the molecular mechanism of what's going on here will be real key to getting this into clinical trials.
Yea, this is potentially a huge breakthrough. A should have caught onto this when the mouse was reproductively cloned Ryuzo Yanagimachi in 2000. They left their SCNT embryos sitting on the bench for ages (under cold and ph stress) before oviduct transfer. I will set a couple of my PhD students to start making human STAP cells – once the Chinese New Year is over.
Thanks to the hard work of my PHD students and Postdocs, we have nearly completed our STAP study. Below you will see some of our results. Enjoy!
Wall Street Journal: Teruhiko Wakayama wants to retract the two STAP papers he co-authored in the Nature journal with Haruko Obokata. He cites there are “crucial mistakes” in the papers making them “no more credibility”.
http://online.wsj.com/article/BT-CO-20140310-703922.html
Http://www.cnn.com/2014/04/01/health/time-stem-cell/index.html?hpt=hp_t2
Stem cell scientist guilty of falsifying data
TIME.com
By Alice Park, TIME.com
updated 2:57 PM EDT, Tue April 1, 2014
http://www.cnn.com/2014/04/01/health/time-stem-cell/index.html?hpt=hp_t2
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