i am isolating EV from culture emdium of amniotic fluid stem cells amd injecting them into damaged ovaries of rats . i was wondering if anyone has tried injecting them into the tail vein and yielded results.
Hi Eman,
This might help:
https://www.nature.com/articles/srep10721
Recently a number of papers addressed this issue, for example
Wiklander et al. Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting. J Extracell Vesicles. 2015; 4: 10.3402/jev.v4.26316.
Info from different sources:
The steady-state level of EVs in circulation reflects a balance between the EV generation and their clearanceHalf-life of purified exogenous EVs, artificially introduced into circulation, is very short: Biotinylated rabbit EVs are cleared in rabbit circulation in ~10 min EVs from splenocyte supernatants, red blood cell-derived EVs and EVs from B16 melanoma cells all showed a clearance of more than 90% after 30 minBiodistribution: red blood cell-derived EVs show an uptake by the liver (44.9%), bone (22.5%), skin (9.7%), muscle (5.8%), spleen (3.4%), kidney (2.7%) and lung (1.8%) melanoma-derived EVs are mainly taken up by lungs and spleen B cell exosomes selectively bind follicular dendritic cells in lymphoid follicles EVs released by a human intestinal epithelial cell line interact preferentially with dendritic cells rather than with B or T lymphocytes.
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