Has anyone tried GoldView (by Dongsheng Biotech) for DNA staining?

More María Celina Zabaloy's questions See All

Which is the most appropriate strain/ clone or DNA for constructing a standard curve for archaeal amoA quantitative PCR?

I need to build a standard curve for qPCR of archaeal amoA using Sybr Green technology. I've seen that several papers use the primers designed by Tourna et al. (2008), and in turn, that paper...

05 June 2015 9,375 5 View

Has anyone dealt with error #1404 in ABI 7500 One Step? It would not allow me to change annealing and extension temperature and duration.

The pre-programmed Annealing/Extension+ plate read step at 60ºC for 1 min is sub-optimal for my qPCR assay (low efficiency, NTC amplification, etc). When I tried to set conditions for my primers...

03 April 2014 9,941 5 View

How could DNA shearing affect qPCR quantification?

I plan to build a standard curve for 16S rDNA quantification via real time PCR by serial dilutions of P. aeruginosa genomic DNA. I extracted DNA using a commercial kit and quality control run by...

02 March 2014 8,101 19 View

What is a plausible explanation for obtaining a plasmid of incorrect size after a miniprep?

I wonder if anyone could help me with a technical problem in this regard. I'm using the pJet1.2 plasmid (from CloneJet, Fermentas) to clone a 491 bp gene in E.coli DH5a. This bacteria holds no...

01 February 2014 6,363 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View