María Celina Zabaloy

5 Questions 12 Answers 0 Followers

Questions related from María Celina Zabaloy

Which is the most appropriate strain/ clone or DNA for constructing a standard curve for archaeal amoA quantitative PCR?

I need to build a standard curve for qPCR of archaeal amoA using Sybr Green technology. I've seen that several papers use the primers designed by Tourna et al. (2008), and in turn, that paper...

06 June 2015 9,330 5 View

Has anyone dealt with error #1404 in ABI 7500 One Step? It would not allow me to change annealing and extension temperature and duration.

The pre-programmed Annealing/Extension+ plate read step at 60ºC for 1 min is sub-optimal for my qPCR assay (low efficiency, NTC amplification, etc). When I tried to set conditions for my primers...

04 April 2014 9,889 5 View

Has anyone tried GoldView (by Dongsheng Biotech) for DNA staining?

I currently use Gel Red and it's great, but also very expensive (about USD 200). Any alternatives? Comments?

03 March 2014 7,306 2 View

How could DNA shearing affect qPCR quantification?

I plan to build a standard curve for 16S rDNA quantification via real time PCR by serial dilutions of P. aeruginosa genomic DNA. I extracted DNA using a commercial kit and quality control run by...

03 March 2014 8,022 19 View

What is a plausible explanation for obtaining a plasmid of incorrect size after a miniprep?

I wonder if anyone could help me with a technical problem in this regard. I'm using the pJet1.2 plasmid (from CloneJet, Fermentas) to clone a 491 bp gene in E.coli DH5a. This bacteria holds no...

02 February 2014 6,312 5 View