I am wondering about the application of FISH on the DNA fiber prepared by your new method. Has anyone tested how signals hybridize using a labeled DNA onto your DNA fiber?
Article Combing of Genomic DNA from Droplets Containing Picograms of Material
Hi Kai.
We haven't tested it, no. The DNA is overstretched when deposited. We have no idea what will happen to hybridised FISH probes during deposition or (alternatively) if they will hybridise post-deposition. The DNA is very loosely bound to the surface, though, so adding solution to it can lead to detachment. Let me know if you try it. I'd be very interested to hear how it works out!
Rob
The overstretch is uniform, so don't expect problems there. I think you'r right that the background (adsorption of FISH probes that aren't hybridised) is going to be the most challenging aspect of the experiment. Not sure on the protocol for FISH though.
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