Dear Meaghan, 

Check these out: 

Monitoring the source of mesenchymal stem cells in patients after transplantation of mismatched-sex hematopoietic stem cells plus third-party cells.

Wang J, Huang XJ, Xu LP, Liu DH, Chen H, Chen YH, Lai YY, Hou RQ, Qin XY, Liu KY. Chin Med J (Engl). 2013 Nov;126(22):4254-9.

Partially mismatched transplantation and human leukocyte antigen donor-specific antibodies.

Gladstone DE, Zachary AA, Fuchs EJ, Luznik L, Kasamon YL, King KE, Brodsky RA, Jones RJ, Leffell MS.

Biol Blood Marrow Transplant. 2013 Apr;19(4):647-52.

No evidence for recipient-derived hepatocytes in serial biopsies of sex-mismatched liver transplants.

Pilat N, Schoppmann S, Stift J, Mazal P, Wekerle T, Berlakovich GA.

Transplantation. 2012 Nov 15;94(9):953-7.

Chimerism analyses by sex-chromosome analysis confused relapse with donor cell leukemia aftersex-mismatched allo-SCT.

Matsukawa T, Shigematsu A, Hayasaka K, Fujisawa S, Asanuma S, Yasumoto A, Goto H, Takahata M, Endo T, Tanaka J, Hashino S, Shimizu C, Imamura M.

Bone Marrow Transplant. 2012 Dec;47(12):1583-4

Hi,

I previously did this for syngenic mice and never notice any particular adverse side effect, no graft rejection. Of course it's on mice only, I have no knowledge with other species.

You probably  mean female recipients. Is your questionabout animal experiment or clinical BMT? Syngeneidc or allogeneic? And for what purpose do you wish to know about this combination?

Hello Dirk -- I clarified the question, thanks for the suggestion. They would be syngeneic bone marrow chimeras made between male and female C57BL/6 mice for the purpose of studying differential effects of the male and female immune response post-injury.

I'm wondering if the female immune rejection of the male bone marrow would make this kind of experiment too convoluted. Thanks for your help!

I have not done male BM to female host, but I think in a ideal chimerism (>95% hematopoietic cells from donor), the host immune system is rebuilt by the male hematopoietic stem cells which give rise to all the immune cells, and therefore the female host will have male immune cells that will not attack the male cells but instead the female stromal cells in the bone marrow and quiescent resident cells elsewhere? So my conclusion is probably you will get rejection.

This is an older post but it doesn't seem like the OP got their answer. We occassionally do bone marrow transplants from male C57BL6/J WT or KO to female C57BL6/J WT or KO recipients, and it goes well. You can check chimerism by measuring Y chromosome copy number. Also, clinically, male to female bone marrow transplants are done successfully in patients, and chimerism can be monitored using XY FISH.