Is there any variation in d dimer levels due to the different methods adopted for analysis?

While doing my research work, I have noticed that the D- dimer levels of normal animals (dogs) are given in different ranges in different publications. Is there any variation occuring in d dimer...

08 November 2020 296 1 View

Is there any ECG database available for different emotions of children with Autism ?

researching in the area of emotion recognition

14 August 2020 8,853 2 View

Can anyone share any qualitative research done specific to non communicable disease (NCD) risk factors in India?

I want to explore specifically Qualitative research done to explore reasons for NCD / socio economic and behavioral determinants of NCD risk factors / perceptions / practices / barriers in care...

27 December 2019 7,816 5 View

Which one is the good software to solve Parametric Mixed Integer Nonlinear Programming?

Hi experts I am using the SCIP solver interface with MATLAB to solve Mixed Integer Nonlinear Programming problem (MINLP). Now, I want to solve Parametric MINLP problem. I am not sure weather...

16 April 2019 5,440 8 View

Can anyone share some matlab simulink file to measure State of Energy (SOE) for Lithium ion battery ???

I am working on power sharing between batteries and i want to take decision on the base of State of Energy (SOE) instead of SOC of batteries. I am looking for some basic or advance algorithm for...

06 February 2019 3,577 10 View

Bovine HbA1c measurement using ELISA Kit ?

09 January 2017 7,152 1 View

What is a influence of substrate temperature on optical energy band gap?

I have deposited ZnO films using spray pyrolysis method, optical transmittance was increased with increase in substrate temperature..so may i know what happens for optical energy band gap? Whether...

07 August 2016 6,418 4 View

Antyone familiar with CRISPR/Cas 9 - gRNA scaffold secondary structure?

Hello, Do anybody face issues or have concerns about the gRNA scaffold's secondary structure formation. During in-vitro transcription, there is no heating and cooling step to enable the formation...

06 April 2015 8,653 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View