Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

What crossover operators can be used for a GA that finds suboptimal positions for a set of buildings?

I'm currently working on my undergraduate thesis where I develop a genetic algorithm that finds suboptimal 2D positions for a set of buildings. The solution representation is a vector of real...

11 January 2021 9,835 4 View

Dear professors, i am confused with how to figure out the tea bag index?

Hello, i am confused with how to figure out the tea bag index,could you please tell me how to compute the letter "a" and "k". Can i use R or Sigmaplot to fix it?

09 December 2020 6,705 3 View

How did Rutherford and Thomson derive the equation for average deflection of a particle hitting an atom?

08 November 2020 9,046 2 View

Converting csv files with data for mapping rainfall on QGIS?

Hi, I am hoping to produce rainfall and temperature maps for a current project in QGIS. I have csv data downloaded which have been imported into QGIS. My aim is to be able to produce rainfall...

10 October 2020 4,930 5 View

Strain Hardening Phenonmenon under Uniaxial Tension when using ABAQUS CDP?

Dear all, I encountered some problems when using the ABAQUS CDP model. Could you please give me some suggestions? I did a monotonic uniaxial test using a single 3D solid element (C3D8R) and the...

04 October 2020 7,111 3 View

How to control variables in a water hammer experiment?

I'm doing a high school experiment on how the impulse of water hammer is affected by pipe diameter, and I'm unsure of how to set up my experiment. My primary concern is safety and validity: - Is...

06 September 2020 1,252 3 View

Buckling Analysis - Risk vs Ex/Implicit vs Static Stabilised - Displacement vs Force Control ?

Dear all, I am studying how to conduct lateral-torsional buckling analysis of a simply supported beam. The beam is purely elastic, and the boundary conditions are shown below. I started with a...

21 July 2020 3,761 2 View

How do I detect the onset of each period of a noisy periodic signal (MATLAB preferred)?

I have a noisy periodic signal, like the attached picture, is there a way to extract the time of each onset of each period (shown by red arrows)? MATLAB preferred other programming languages also...

16 July 2020 5,988 8 View

Incorrect simulated conductance value compared to analytical solution using ANSYS Maxwell 2D?

31 May 2020 7,991 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View