Has anybody had any contamination like this within their organoid cultures before? Or does anyone know what might be contaminating it? Thank you.
Would anyone be able to advise me... I have an RCT with a control and experimental group. Participants were recruited from one school (n=59). Participants were assessed using repeated measures on...
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I have written and posted several articles on the Designing Buildings website. I now wish to add some of them to my profile on ResearchGate. However, I have tried numerous times to upload the...
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I thought the zero divisors were the polynomials of the form ax^2+bx+2c where a,b,c=0 or 1. But I've since figured out that this quotient ring is not local, so there exists other zero divisors...
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looking for discussions and examples of cyber attacks on operational technology in any industry
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Does anyone know of any laboratory measurements demonstrating the nonlinear pulse excitation of magnetoplasmas? I did a thesis back in 1971 using baseband high voltage picosecond pulses showing...
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Translation and Cultural Adaptation principles internationally offer accepted principles in regard to existing instruments to be translated in other languages after getting permission from the...
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Dear experts, I am running longitudinal models using gamm4 with interest in looking at main effects of group and group by age interactions (age range 8-25 years). I am using the following...
13 February 2023 1,815 0 View
I need to examine soft tissues in the mucosa around the skull as relates to intracranial stuff. So keeping that stuff intact and in place is essential. There's a zillion protocols around - I'm...
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Due to the limited number of studies on the RA of MP in freshwater, I stuck on the contributing factors and development of RA model. Is there anyone doing the same topic?
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I am setting up an RT-PCR experiment for the first time and I am trying to get the calculations for making up my working primers. This is what I have so far. Forward primer: initial...
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
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Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
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Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
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I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!
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Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...
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I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
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The question refer to leafy biomass decomposition using litterbag techniques.
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There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...
11 July 2024 4,829 0 View
When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?
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