What statistical test to use in SPSS to investigate relationship between continuous variable and national mean for another variable?

I am exploring the readability of court decisions in 5 countries. I have a quantitative readability measure (continuous variable, my dependent variable) for each decision - about 50 decisions per...

08 February 2021 419 3 View

How are the degrees of freedom calculated for a bootstrapped Independent Samples t-test?

I am currently writing up findings for my masters dissertation and I have noticed that APA often require the t statistic, the p-value, degrees of freedom and sometimes the confidence intervals...

08 August 2020 5,367 3 View

What am I doing wrong in molar solution calulation?

I recently read: "The perovskite solution for the solvent quenching method (MAPI) recipe was prepared by dissolving 553.2 mg PbI2 and and 181.23 mg MAI in 1 ml anhydrous DMF/DMSO (4:1, v:v) to...

17 May 2020 9,876 7 View

What are the molecular determinants for formation of adducts in electrospray mass spectrometry?

Dear all, depending on the molecular structure of an analyte different adducts during the electrospray process are formed. Even when high (up to 5 mM) of ammonium salts are present in the LC...

13 May 2020 6,621 3 View

How to do coordinate transformation of a 6x6 stiffness matrix of tilted transverse isotropic medium?

Tilted transverse isotropy (TTI) is sometimes treated as a rotated vertical transverse isotropy (VTI) rock (Figure 1). I have the 6x6 stiffness matrix of a rotated VTI as in the following Figure...

03 April 2020 1,404 18 View

How can we compute pore pressure from post-stack seismic section?

I have read most publications which suggest computing pore pressure from pre-stack seismic section, through AVO. Otherwise, if we have post-stack seismic section, how can pore pressure be...

19 January 2020 6,664 3 View

What could have created the first and second bands in the third and fourth lanes from the right?

I am an undergraduate biochemistry student. This is my first time doing DNA agarose gel electrophoresis. The third and fourth lanes from the right are loaded with samples obtained from E. coli...

09 March 2019 3,225 3 View

How to make a simple perovskite diode without HTL and ETL?

I am facing trouble in making a simple perovskite diode in order to study the deep level traps of the perovskite semiconductor layer. Since the properties of perovskite layer are needed to be...

20 January 2019 2,593 4 View

Are there any Cryopreservants/Cryoprotectants with no DMSO?

Hello there, I'd like to ask whether there are any cryoprotectants/cryopreservants medium with no DMSO in them. Specifically to use it for freezing tissue such as collagen scaffold. I have found...

12 November 2018 8,949 3 View

Does anyone titrate their secondary antibodies for intracellular flow?

Hey everyone, I'm working on creating a flow QC panel. I have a few sets of 3 intracellular and 3 surface markers that I know have worked well in the past for ICC and flow applications. My...

27 September 2018 6,166 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View