We are doing amino acid analysis through sample derivatization by OPA, but few amino acids ( Hydroxyproline & Proline) are not detectable by the same. So, we would like to use combinedky the FMOC and OPA for sample derivatization. We are using FLD detector for amino acid analysis.
Dear Maity
I also analyzed amino acids but i ll prepare these chemicals always separately and also keep separate vessels to prepare the same. Because, FMOC, OPA and MPA they are most sensitive if any tiny contamination while preparing these chemicals, which lead more negative influence on the final result and most of the time, the peak itself will not be proper. My better suggestion, they have to be prepared always separately. However, i did not try my mixing the same together during my research period.
Dr. Jannathulla... Is this possible to mixed FMOC and OPA before injecting to HPLC?? ... I need to identify the Hydroxyprolin and proline which only possible thorugh FMOC derivatization
I suggest moving away from derivatizations(huge variability, ancient technique) and use direct detection like HPLC-CAD.
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