We have a QIAcube HT and I can't seem to get consistent yield/quality out of it when isolating gDNA from CD4 T cells. We've moved to an off-board lysis in the deep-well 96 plate using buffer AL (from the QIAamp kit), proteinase K, and RNase A, at 56C with shaking, before loading the lysate onto the QIAcube HT for subsequent column binding/washing/elution - but even with the off-board lysis we get wildly variable yields and inconsistent quality (260/280, residue). We can't use the TopElute feature due to incompatibility with downstream applications.
Does anyone have a custom protocol/tricks that have worked well to improve the QIAcube's performance here? We are hesitant to go back to doing the sample lysis in individual tubes (with our current protocol or the TissueLyser) since at that point, what's the point of the QIAcube HT?
Appreciate any thoughts!
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