I am trying to find a suitable medium for the liquid culture of Faecalibacterium prausnitzii. Looking through the literature and based on recommendations from others, I think I should use YBHI (supplemented BHI) media. However, the method for making this is generally quite vague (see below). Has anyone made this before and found it has worked for culturing F. prausnitzii? Could you provide more detail as to how you made the media? e.g. can cellobiose and maltose be autocaved? Can L-cysteine and resazurin be included? Was the media sparged with O2-free N2 prior to autoclave?

"F. prausnitzii A2-165 (DSM 17677), isolated from human fecal stool, was grown at 37°C in YBHI medium (brain-heart infusion medium supplemented with 0.5% yeast extract [Difco]) supplemented with cellobiose (1 mg/ml [Sigma]), maltose (1 mg/ml [Sigma]), and cysteine (0.5 mg/ml [Sigma]) in an anaerobic chamber". From Miquel et al, mBio Apr 2015, 6 (2) e00300-15; DOI: 10.1128/mBio.00300-15