I did as follow. I mix 1ml of starch with 1ml of crude enzyme. incubated for 15 minutes. After that I added 1ml of DNSA and bioled for 10 moinutes. after boiling i added 5ml of distiled water. thes measured absorbance at 540nm. my maltose standard curve equation is y=0.0009x + 0.01. how can I calculate Enzyme activity?
Dominique Liger
Hi there,
The first issue would be to get a much more accurate equation for you standard curve. The slope has only one significant number (what are at least the 2 first numbers after the 9 ?)... Then reporting your readings onto the curve would give you the corresponding maltose amount/concentration produced in 15 minutes per mL of crude enzyme.
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