Does anyone know providers of small (< 1um) soft particles e.g. polyethylene?

Hello, everyone! I am looking for providers of small soft particles (polyethylene, polystyrene) with sizes around 1 micrometer or less (it could be even better). I looked through the Internet, but...

14 December 2020 4,217 1 View

Can you recommend me a good online course about molecular biology? (Other biomedical online courses as well) ?

Dear Scientists Due to serious pandemic threat and the "stay at home" recommendation, I would like to ask you for recommendations of good online biomedical courses. My focus is to get better with...

15 March 2020 4,955 9 View

Why crystallization stops at melilite - pseudowollastonite cotectic?

29 January 2019 3,981 2 View

What is the reactivity of 2-aminoimidazole?

Can anyone say something about the reactivity of 2-aminoimidazole? I think this compound significantly differs in term of chemistry from other heterocyclic amines, e.g. 3-amino-1,2,4-triazole,...

17 January 2018 7,331 3 View

What could be a proper HPLC mobile phase for 3,5-dinitrosalicylic acid analysis?

21 August 2017 429 2 View

What should i change to improve the quality of polyacrylamide gel?

26 February 2017 3,339 9 View

Best conditions for agarose gel electrophoresis to get high resolution?

26 January 2017 3,633 19 View

Can anyone help with pressure gradient in boundary layer profile?

Dear colleagues, Does anyone have a clue how to introduce pressure gradients effects into boundary layer profile definition? So far I was implementing boundary layer profile with zero pressure...

16 April 2015 8,439 4 View

What is the most efficient and fast way of live trapping of Norway rats?

I need to trap alive dozens of wild Norway rats for my research. What kinds of places and what ways of live trapping would you recommend? I have used many kinds of traps and baits and I have tried...

21 January 2015 7,949 5 View

Is there any software that allows for identification of individual animals by their body weight?

19 January 2015 5,272 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View