I am quite adapted to observe multiple peaks at heterozygous sites of nuclear loci, but not in chloroplast loci; does it mean polyploidy? The peaks are not spurious as observed in multiple individuals of same species and more than half the height of the taller peaks.
Balaji Chattopadhyay
do you expect polyploidy in chloroplast?
and is there a possibility of pseudogene? for us generally a double peak in orgenalle genes imply a pseudo gene. We check the appropriate reading frames for stop codon and in the absence of the same, go with a degeneracy at that base for eg.
0 votes
0 thanks
Eitan Rubin
Could be a heteroplasmy with a deletion. Checkout the Child server we developed or contact me directly.
http://nar.oxfordjournals.org/content/early/2011/01/27/nar.gkq1354.full
0 votes
0 thanks
- Badges
- Science topic
More Avik Ray's questions See All
Similar questions and discussions