Hello,
I use X-ray microtomography method to assess vascularization within a motor cortical transplant from cortical lesion of murine model. To do this, I perfuse NaCl, PFA and a contrast agent to visualize blood vessels. But when I pass the brains to the X ray microtomograph, it is impossible to visualize the different structures of the brain in order to overlay the immunohistochemistry slices with microtomography slices.
I did some research and i found informations about phosphomolybdic acid and phosphotungstic acid which are contrast agents for tissues, but these products are used on muscles and systemic veins. (Nierenberger et al., 2015 and Pauwels et al., 2003)
Does anyone have any information about these products applied to brains?
Thanks !
Dear Antoine, I think this to be a very interesting project.... admitting that I am not working with nor have ever done X-ray-microtomography... but found your question interesting (not only because I am "FRMS")... so only I searched for appropriate literature or recent articles that eventually hit (might meet) your expectations: e. g.:
"Soft tissue discrimination with contrast agents using micro-CT scanning", DESCAMPS et al: Belg. J. Zool., 144(1) : 20-40, Jan. 2014; pdf @ http://www.fun-morph.ugent.be/sites/default/files/Descamps%20et%20al%202014%20-%20BJZ%20-%20CT%20soft%20tissue.pdf (open ACCESS);
"Non-invasive imaging of transplanted human neural stem cells and ECM scaffold remodeling in the stroke-damaged rat brain by 19F- and diffusion-MRI", BIBLE et al, Biomaterials, Vol.33, Iss.10, April 2012, Pages 2858–2871 (unfortunatelyPPV=pay per view) @ http://www.sciencedirect.com/science/article/pii/S0142961211015031.
Another approach (microfil-injection) in: "RALBP1/RLIP76 Depletion in Mice Suppresses Tumor Growth by Inhibiting Tumor Neovascularization", by LEE et al, 2012, Cancer Res; 72(20); 5165 –73. pdf free access @ http://cancerres.aacrjournals.org/content/canres/72/20/5165.full.pdf.
"Diffusible iodine-based contrast-enhanced computed tomography (diceCT): an emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues" by GIGNAC et al, 2016, in J. Anat,Vol.228, Iss.6, June 2016, Pages 889–909, open access @: http://onlinelibrary.wiley.com/doi/10.1111/joa.12449/full or the pdf @ http://onlinelibrary.wiley.com/doi/10.1111/joa.12449/epdf (they cite PAUWELS et al, 2013 [not 2003] : Pauwels E, Van Loo E, Cornillie P, et al. (2013) An exploratory
study of contrast agents for soft tissue visualization by means of high resolution X-ray computed tomography imaging. J Microsc 250,21–31.
There are perhaps some more interesting articles...
HUTCHINSON et al, 2017, "Early clinical applications for imaging at microscopic detail: Microfocus Computed Tomography (micro-CT) cf: http://www.birpublications.org/doi/abs/10.1259/bjr.20170113 [unfortunately only PPV);
TAHARA & LARSON, 2013: "Quantitative analysis of microscopic X-ray computed tomography imaging: Japanese quail embryonic soft tissues with iodine staining", J. Anat, Vol.223, Iss.3 September 2013,Pages 297–310, @ http://onlinelibrary.wiley.com/doi/10.1111/joa.12081/full or pdf (open access): @http://onlinelibrary.wiley.com/doi/10.1111/joa.12081/epdf .
I stop here hoping that reading any of the mentioned articles might help you with your problem. Best wishes and good luck, WM.
This important link
http://www.fun morph.ugent.be/sites/default/files/Descamps%20et%20al%202014%20-%20BJZ%20-%20CT%20soft%20tissue.pdf
Thank you a lot for all of this article.
I searched also for articles and i found this :
Volume Shrinkage of Bone, Brain and Muscle Tissue in Sample Preparation for Micro-CT and Light Sheet Fluorescence Microscopy (LSFM)
Jan Buytaert, 2014 (https://www.ncbi.nlm.nih.gov/pubmed/24963987)
It seems that PTA causes shrinkage of biological tissues due to ethanol treatment. About 30% of the volume.
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