Has anyone ever done ELISA for total MTOR content from plasma? If so, then how do the results compare from those done from cell lysate?

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Ben Stevens

well, considering that mTOR is an intracellular serine/threonine kinase I would not expect any mTOR in plasma.

Parag Palit

But then again, there are several reputed kit manufacturers who have commercial ELISA kits for mTOR from plasma. However, none of those kits have any citations.

Karen A. Darbinyan

Hello, see attached info may be useful for you.

Good luck.