Has anyone encountered a double digestion problem for restriction mapping?

10 October 2014 1 5K Report

This double digest problem is used for restriction mapping. In this problem I am using two different enzyme to cut the strand of DNA individually or combined both. Can anyone suggest how to map the location of cut site in this problem?

Ehab El-Nahas

Hi Ashwani, my recommendation to you, is to read the paper by Hepfer, C. E and Turchi, S L. 1989. An Introduction to Restriction Mapping of DNA. Biochemical education 17(1). your problem will be solved.

regards

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