Hi,

Nice to discuss with you. I know this kit but I just do this assay in 48-wells plate by myself following 23-day culture protocol with the soft agar gel.

But what you want to know?

Thanks for your answer, I just want to know this kit worked fine for your experiment. 

I tried one time it worked but it really depends on your cell type. I can tell you that  MCF7  cells  grow  quite well. 

Just follow the protocole of the kit and you can have your result after one week. 

Thank you so much 

You are welcome. Good luck!