I've tried many solvents and precipitation agents but still don't know which one would serve the best, bearing in mind that glutathione is a labile compound and changes its concentration rapidly.
Hello, why without derivatisation? do you mean extraction before derivatization?
Hi! I mean an extraction technique from plant to determine glutathione level but as a non-derivatised substance. There are many publications concerning glutathione determination with derivatisation but I want to extract it and derermine it, without changing its structure. I already have a method on my LC-MS/MS for glutathione so now I need a plant extract with it. Do you know what I mean? Is it possible? Thanks:-)
Hi, I don't have data on the stability of GSH in plant extracts but in tissues and biological fluids post-extraction derivatization is a must because the GSH is oxidized into GSSG quite fast. Derivatization with e.g. NEM (N-ethyl-maleimide) can be very straightforward and you could also use LCMS/MS for measuring GS-NEM
Hi, thank you very much for your answer. I'll try to find the best method.
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