Several labs from different countries  had troubles to reproduce this method and already wrote to Nature Biotechnology to request  authors to publish their raw data.

Some people suggest to give up  and not waste time to try.

Hello Junming

Thank you.  i am well aware of the reported cases of so-called success and the scores of reports that it does not work. Have you tried this in cells or , even better, mice?  And what exactly were the parameters?

We are trying and our first round of injections came up negative on a target we previously publish dusing CRISPR

Hi Joseph,

The paper from [http://www.nature.com/nbt/journal/v34/n7/full/nbt.3547.html] is currently highly disputed, as you are aware. I have not seen a publication from any group who has published anything on NgAgo genome editing in cell lines (except for the paper above :-) ) or mice. We were also about to do NgAgo genome editing in blastocysts and mice, however we paused this as it is probably not going to work at this point (as noone can even reproduce the in vitro results from the paper ..).

By the way, out of curiosity, are you first going to perform an in vitro digest with your NgAgo, DNA guide and amplicon of your target site? 

Hi, Joe,

We are not working on NgAgo system.  Here are recent  comments on this system.

http://www.nature.com/news/replications-ridicule-and-a-recluse-the-controversy-over-ngago-gene-editing-intensifies-1.20387

Thanks Emiel.  No, we go right into the mouse with a codon optimized version 

SO far no success.  It's probably not going to work as advertised.

Junming,  I have that article on my office door LOL