Emiel ten Buren

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Questions related from Emiel ten Buren

Options for CD4+IFNg+ murine T cell lymphoma cell line?

Hi guys, Have any of you come across a murine T cell lymphoma line that has constitutive CD4 expression and IFNg expression (or IFNg expression upon e.g. PMA/Iono stimulation) ? Or any other...

23 May 2018 8,093 0 View

Mutation in donor template homology arm: (how much) will this impact homologous recombination?

Hi guys, I am going to knock-in a donor template (HA-left // transgene cassette // HA-right) into a CRISPR/Cas9-mediated DSB. Homology arm (HA) left = 3.5kb Transgene cassette = 6.5kb Homology...

21 November 2017 4,717 1 View

Does anyone know an antibody for detecting via flow cytometry MS2-p65-HSF1?

MS2-p65-HSF1 is a transcriptional activation domain. By binding to aptamers from sgRNA2.0 (see Konermann 2015, Nature) you can target it with dCas9 to regulate expression of a gene of...

22 February 2017 7,696 0 View