Has anyone been able to successfully cryopreserve coral oocytes?
Though protocols for coral sperm cryopreservation have been established (Hagedorn et al., 2012; 2017; 2018; Daly et al., 2018 and others), these have not been successful for stony corals' oocytes, I've only found one positive research for a Gorgonian (Junceella juncea) through vitrification (Tsai et al. 2015, attached below). I know that in some cases some researchers (personal communications) have been able to thaw and retrieve the eggs from liquid nitrogen, but even though they are still 'alive', they loose their ability to be fertilized (after thaw and reactivation sperm will not fertilize them). Has this happened to you too?
I’m trying to see what we can implement in order to succeed at this matter, for if we expect to achieve genetic biodiversity conservation for corals and other sexually propagating species, banking only the sperm might not be enough, though I know there has been some advances in cryopreserving larvae.
I would appreciate it very much if there’s anything you think you could share with me or maybe recommend that could give me some further insight into this matter.
Thank you
Article Development of Cryopreservation Techniques for Gorgonian (Ju...