I am attempting to grow biofilm from the anaerobic granules I took from a wastewater treatment plant. I crush them using a syringe and inoculate it with lactate (and growth media). I am currently using a drip flow reactor and various coupons but so far, I have not manage to see visible biofilm growth.
Batch mode is 48 hours and continuous flow is set at 0.2ml/min for 120 hours (5 days). Does anyone have an experience with this? Is the incubation time to short?
I have experienced growing biofilm from mix culture bacteria, it will be much easier if you added some bedding material as it will be good 'house' for bacteria/ microbes. May be you need to add some co-nutrient to increase biofilm formation, in my case I used Nutrient Broth added to wastewater and that help alot.
I hope you are trying these things in anaerobic conditions. Aerobic condition going to hamper the culture and process of development. Bio-film formation is different from Sludge culture. Sludge acting as solid but porous support, so its like a filter where culture gets trapped. It may not possible to convert all of them in bio-film form. As its is completely culture property. Better you go with small UASB reactor.
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