I'm outlining an experiment where you'd need to fix peptides in a 96-well plate. And now it would need some method or even the protocol for it.
Hello Marcel,
I think following articles would help
Biopolymers. 2013 Apr; 100(2): 167–173.
doi: 10.1002/bip.22188
Anal Biochem. 2004 May 15;328(2):162-5.
DOI: 10.1016/j.ab.2004.02.003
http://dx.doi.org/10.1155/2011/421701
Also, there are some plates specifically produced for binding amine to plates, Please see below:
Well-Coated™ Amine Binding plates are maleic anhydride activated plates that react with primary amines to form amide bonds that are stable at pH≥7. Acidic conditions will hydrolyze the bonds releasing the peptide/ligand, therefore binding of peptide/ligand to plates should be performed at pH8-9 and the binding assays or ELISA should be performed at pH≥7.
adopted from
https://www.gbiosciences.com/Protein-Research/Protein-Detection-Analysis-Systems/ELISA/Coated-Plates/Well-Coated-Amine-Binding
Hope it will help
Amin
Hello Marcel,
I attach you the protocol I used in the paper 2006JMV (in my contributions), it is in spanish, any doubt, do not hesitate in contact me again. The brand of the plate and its efficiency is very important, I had differeces among brands and had to use the mor expensive plates, but the worked well.
Good luck!
Celeste
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