Why should anyone use 2D cell cultures for cancer research (Apart from 3D cultur, GEMMs, etc.)?

I have to discuss the question, why anyone should still use 2D cell culture, as this tumor model seems to be the worst of all models. Do you see any advantages?

02 March 2016 4,503 5 View

Can anyone help with establishing colorectal cancer cell lines?

What medium do you use? Any other good tip for me (coated wells, etc.)? PS: We are trying to establish primary cell lines from metastases.

09 October 2012 3,594 11 View

What is the precondition for successful isolation of cell lines?

Isolation of cell cultures is only possible in 10% of the cases. Most tumor samples do not show outhgrowth. Does anyone know why? Are there studies on the involvment of certain mutations within...

08 September 2012 1,954 12 View

Do you know what the underlying problem of this patient is?

Female patient in her 50s. Since she turned 18 she's has multiple episodes of cholangitis and colic. cholangiolithiasis (pronounced) and choledocholithiasis. The gallbladder was removed 30 years...

08 September 2012 3,417 6 View

Recently we published a hypothesis on early carcinogenesis. I would be interested if there is a possibility to computationally identify other interc...

Recently we published a hypothesis on early carcinogenesis. I would be interested if there is a possibility to computationally identify other intercellular feedback loops?

05 June 2012 3,382 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View