Has anybody tested H4K20me3 antibodies for specificity in ChIP assays (specificity in terms of no cross-reactivity with H4K20me2) ?

27 March 2019 1 5K Report

I have found in preliminary tests that many (6 tested so far) of the commercially available H4K20me3 antibodies can be blocked in ChIP assays by K20me2 peptides as well as K20me3.

Has anybody had similar experiences with other histone modifications like H3K4me2/3 or H3K9me2/3 etc? Come to think of it - most ChIP-seq studies show quite similar profiles for KXme2 and KXme3 histone modifications while KXme1 tends to be less overlapping.

Baida Ali

We have not tested the interaction mentioned aboveز

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