Has anybody had success patenting a Mendelian disorder hiPS cell line?

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It was published in the Journal of the American College of Toxicology in 1990, but the author's information does not appear in the journal

29 November 2020 2,554 3 View

Has anyone been successful in seeding microglia from adult mice?

Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...

14 October 2020 4,572 3 View

Can I use the cryostat to slice brains that were perfused with 4% PAF and Gadoteridol?

Hi everyone Im trying to slice my mice brains with the cryostat, however, I haven't been successful, the tissue seems to stretch and then it breaks. Let me tell you the story about these brains:...

25 August 2020 6,479 4 View

How to transfect Tcells with siRNA?

Hi everyone, I am trying to transfect murine CD4+ T cells in vitro, using Lipofectamine RNAi/MAX however, even when trying troubleshoot, the positive FITC labelled control shows just about 3%...

03 June 2020 2,618 1 View

Alternate morphology of eletrocardiographic signals after heart mechanical injury..... ???

Hello, I need your help. We are studying electrocardiographic parameters in an experimental model of heart injury, and we obtained unexpected results. The ECG intervals alternated during the...

28 September 2017 2,581 4 View

What can I do to avoid contamination of my nested pcr?

Good day, I try to make a pcr nested but I have problems of contamination, I use as a template the negative product of the pcr in the second pcr and gives me positive. I thought that the pollution...

24 January 2017 6,992 2 View

Is there any reliable antibody against corticotropin-releasing hormone (CRH) for Western blot?

Hi guys, does anybody know any CRH antibody which would work for western blots giving a nice and specific signal, especially in brain tissues (PVN, hippocampus)? Any advice is welcome. Thank you.

27 January 2016 320 3 View

Sophie's choice: Random mate-pairs or gel selection in Illumina Nextera mate-pair preparation?

Hi guys! I'm sequencing and assembling a de novo invertebrate genome of about 0,8Gb. I want to make a lane run of Illumina nextera mate-pairs, but I'm having some difficulty on selecting the size...

06 January 2015 6,134 9 View

Can anybody recommend a good and specific p-CREB and CREB antibody for westerns in rat brain tissue samples?

Thanks in advance.

22 February 2014 7,262 4 View

The most appropriate nuclear and cytosolic fraction housekeeping gene?

Could anyone recommend the most appropriate housekeeper for nuclear and cytosolic fraction? I want to isolate both fractions from frozen rat brain tissues and am wondering what is the best and...

05 August 2013 8,453 6 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Any suggestion on literature in Cyber Crime against children?

dear all I am looking for on literature in Cyber Crime against children.

11 January 2021 5,099 5 View

Does the initial cell number play a role in affecting the output variables like IC50, EC50, etc.in cell-based drug screening assays? If so, how?

While performing cell-based assays to assess drug toxicity or drug efficacy, how is the initial cell seeding density determined? What is the standard followed in the pharmaceutical industry while...

07 January 2021 6,983 3 View

Is it necessary to validate the control test for each FFP separately?

Hi everyone, I would like to ask if it is necessary to validate the control test (eg sub-visible particles) for each Finished Pharmaceutical Product (FPP) separately (using the same method, the...

06 January 2021 5,568 3 View

Excipients for moisture, heat and light sensitive API with poor flow properties?

I need to formulate a water dispersible tablet with an a highly potent (Dose 7mg) API which is moisture, heat and light sensitive API with poor flow properties. Please advice on the formulation,...

01 January 2021 1,284 2 View

Why does drug release decrease from the coat of fixed dose combination?

I am developing a Fixed Dose Combination dosage form, which have an active pharmaceutical ingredient in the coat and another one in an extended release core of tablet. During development, I have...

17 December 2020 3,267 2 View

Today, which EU country is leading in the field of sociology of law?

In academic and professional level.

27 November 2020 4,836 8 View

Acesulfame Potassium and Acesulfame and Conversion?

Dear to whom it may concern, Based on ChemAxon, I calculated pKa values of Acesulfame as follows: 1. pKa (Strongest Acidic): 3.02 2. pKa (Strongest Basic): -6 Because I would like to convert...

25 November 2020 4,577 3 View

Arturo Geigel

I did some patent searches and found some patents in IPS are from 2012- 2014. The focus of these patents is on preparing or the production of HIPS not on patenting the HIPS themselves.

Hope this helps

Lukas Dreveny

You can not achieve a protection for cell line such as. In patent application you have to prove the effect of your invention. You could achieve protection on new way of cultivation or production or usage of this cell line for treatment of ... . The most popular system in achievement the patent protection called "problem and solution". You have some problem and your invention offers a solution.